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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

Fe3 Reduces the Titer of bet-Deficient FFV and Induces Genome Editing. The effect of fe3 coexpression with WT and bet -deficient FFV genomes was studied after transfection of。

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。

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